Prevalence and demographic, substance use, and mental health correlates of fasting among U.S. college students

Fasting is an unhealthy behavior that has been frequently used as part of weight loss attempts. To date, little research has been conducted to determine the prevalence and substance use and mental health correlates of fasting among college students. Therefore, the aim of this study was to estimate the prevalence and associations between any (≥ 1 time) and regular (≥ 13 times) occurrences of fasting in the past 4 weeks and substance use and mental health correlates among a large sample of college students from 2016 to 2020. Data from four academic survey years (2016–2020; N = 8255) of the national (USA) Healthy Minds Study were analyzed. Unadjusted prevalence of any and regular fasting by survey year and gender was estimated. Multiple logistic regression analyses were conducted to estimate the associations between any and regular fasting and the demographic (age, body mass index, race/ethnicity, sexual orientation, highest parental education), substance use (cigarette use, marijuana use, other illicit drug use, alcohol use), and mental health (depression, anxiety, eating disorder symptoms, suicidal ideation, non-suicidal self-injury) correlates. Any fasting in the past 4 weeks was common among both men (14.77%) and women (18.12%) and significantly increased from 2016 (10.30%) to 2020 (19.81%) only among men. Regular fasting significantly increased among both men and women from 2016 (men: 1.46%; women: 1.79%) to 2020 (men: 3.53%; women: 6.19%). Among men and women, both any and regular fasting in the past 4 weeks were associated with higher odds of all mental health symptoms, including a positive depression, anxiety, and eating disorder screen, suicidal ideation, and non-suicidal self-injury. Among women, but not men, any and regular fasting in the past 4 weeks were associated with higher odds of marijuana use and other illicit drug use (e.g., cocaine, ecstasy). The results from this study underscore both the high and increasing prevalence of fasting among a national sample of college students, as well as the substance use and mental health symptoms associated with this behavior. Healthcare professionals both on and off campus should consider screening for fasting behaviors among college students and provide appropriate intervention when needed. Fasting has recently garnered public attention given its purported benefits, including weight loss and improved physical functioning. To date, little research has been conducted to determine the prevalence of this behavior among college students, as well as what demographic characteristics, substance use behaviors, and mental health symptoms are associated with this behavior. Results from a sample of over 8000 college students from four survey years of the Healthy Minds Study show that fasting is common among college men and women and has increased in prevalence from 2016 to 2020. Fasting is associated with indicators of poor mental health including depression, anxiety, eating disorder symptoms, suicidal ideation, and non-suicidal self-injury (e.g., cutting, burning), among both college men and women. Fasting is associated with substance use behaviors, including marijuana use and other illicit drug use (e.g., cocaine, ecstasy), among college women. These results highlight the adverse correlates of this common weight loss and compensatory behavior among college students.


Introduction
Fasting, the process of abstaining from food intake for extended periods of time, has grown in popularity over the last several years. The process of fasting, which can include alternate day fasting, periodic fasting, and timerestricted feeding, has been shown to have physiological consequences and elicit changes to the metabolic, cellular, and hormonal processes of the body [1][2][3]. While primarily used as a mechanism for weight loss or the modification of one's body shape [1], some research has pointed to non-weight-related health benefits of fasting, such as improved physical and mental performance [2,3], which may also explain its popularity. To date, research regarding the effects of fasting has primarily focused on testing whether fasting is an effective weight loss mechanism [4,5], as well as identifying the potential benefits to the brain [6], and for reducing cancer [7,8], cardiovascular disease, and diabetes [9,10]. This existing research has left little known about the adverse correlates of this behavior.
Despite the interest in their potential health benefits, fasting, caloric restriction, and dietary restraint behaviors are associated with psychopathology, including suicidal ideation and suicide attempts [11,12], non-suicidal selfinjury [13,14], and anxiety disorder and symptoms [15], as well as clinical and sub-clinical eating disorder symptoms. For example, the cyclical nature of fasting and as a period of unsustainably restricted eating may reduce cognitive control and promote binge eating behaviors [16,17], and among both college women and men, dietary restraint was shown to be a primary predictor of eating pathology [18]. However, it is important to note that, while commonly used as a method to modify body shape and weight, fasting behaviors may be used by individuals pursuing different appearance or weight related goals, which may be differentially related to psychopathology. For instance, fasting may be used to pursue weight loss or to compensate for binge eating [19], or, more recently, fasting may be linked to growing trends promoting biohacking approaches to altering the body to improve physical and mental performance [20,21]. Previous work has shown that, in particular, appearance goals may differ according to gender [22]. Thus, delineating the correlates of fasting among college men and women separately may offer important insights into the respective risk profiles.
To date, little research has been conducted to investigate fasting and its associations with substance use and poor mental health symptoms among college students. However, this age group is particularly vulnerable to engaging in fasting, as well as using substances and experiencing poor mental health symptoms. For example, eating disorders [23,24], dieting, and disordered eating [25] regularly peak in later adolescence and young adulthood. Similarly, body dissatisfaction, a major driver of fasting and related disordered eating, is common among college age young adults [26]. College age young adults are also particularly susceptible to poor mental health, including depression, anxiety, suicidal ideation, and nonsuicidal self-injury [27], as well as have increased access to alcohol and illicit drugs thus leading to substance use [28,29]. Moreover, it is well established that eating disorders and disordered eating behaviors are connected with comorbid substance use and poor mental health [30][31][32][33]. The potential associations between fasting and substance use and poor mental health symptoms may be driven by the overlapping emotion regulation difficulties spanning these behaviors [34][35][36]. The combination of these factors indicates that further research is warranted among this age group to understand the connections between fasting and substance use and symptoms of poor mental health. Therefore, the aims of this study were, first, to estimate the prevalence and demographic correlates of fasting from 2016 to 2020, and second, to estimate the associations between fasting and several measures of substance use behaviors, including cigarette use, marijuana use, other illicit drug use, and alcohol use, and symptoms of poor mental health, including depression, anxiety, eating disorder behaviors, suicidal ideation, and non-suicidal self-injury, among a large, national sample of college students. While there are likely bi-directional relationships between fasting and substance use and poor mental health, we focus specifically on whether fasting, as the independent variable, predicts substance use behaviors and poor mental health. This decision was made a priori given that fasting appears to be increasingly prevalent for purposes of weight loss and body shape control, as well as for the purpose of improving physical and mental performance [1][2][3]. Given the previously reviewed literature, we hypothesized that prevalence of fasting will increase from 2016 to 2020 and will be associated with all indicators of substance use and poor mental health among both college men and women.

Methods
We analyzed pooled data from four academic survey years (2016-2020; N = 8255) of the national (USA) Healthy Minds Study (HMS) [37]. HMS is an annual, repeat, cross-sectional study of several biological, psychological, and social health domains, as well as service utilization, among college undergraduate and graduate student participants. Colleges and universities voluntarily elect to participate in HMS and participants complete three standard modules, along with additional elective modules selected by their institution. At institutions with ≥ 4000 students, 4000 students are randomly recruited to participate in the survey; at institutions with < 4000 students, all students are recruited. To be eligible to participate, students must be at least 18 years old. Students are invited to participate via email and the survey is administered online via Qualtrics. Students are incentivized to participate using a drawing for Amazon gift cards. HMS is conducted out of the University of Michigan and Boston University, is approved by the Health Sciences and Behavioral Sciences Institutional Review Board at the University of Michigan, and informed consent was obtained from all participants [37]. This study was exempt from research ethics board approval given that HMS is publicly available and relies on secondary use of anonymous data.

Measures
Fasting was measured using the question, "Over the past 4 weeks (28 days), how many times have you fasted (intentionally not eaten anything at all for at least 8 waking hours)?" This question is part of the widely used Eating Disorder Examination Questionnaire (EDE-Q) [38]. Given the significant positive skewness (7.5) and kurtosis (126.6) of the fasting variable, and to align with previously identified clinical cut-off points, responses were dichotomized into any (zero [0] times and one [1] or more times) and regular (0-12 times and 13 or more times) fasting, as has been done in prior research [39][40][41].
Cigarette use was measured using the question: "Over the past 30 days, about how many cigarettes did you smoke per day?" This variable was converted into a dichotomous variable with participants who reported zero (0) cigarette use and those who reported any cigarette use [42,43].
Illicit drug use was measured using the question, "Over the past 30 days, have you used any of the following drugs?" Potential response options included: "Marijuana;" "Cocaine (any form, including crack, powder, and freebase);" "Heroin"… Two dichotomous variables were created to investigate illicit drug use: Marijuana use and other illicit drug use. Marijuana use included those who reported any marijuana use and those who reported none. Other illicit drug use included those who reported any illicit drug use (minus marijuana use) and those who reported none.
Alcohol use was measured using the question: "Over the past 2 weeks, did you drink alcohol?" A dichotomous "yes" or "no" response was available.
Depression was measured using the Patient Health Questionnaire-9 (PHQ-9). This screening tool is based on the nine Diagnostic and Statistical Manual for Mental Disorders (DSM)-IV criteria for a major depressive episode and includes questions such as "Over the last 2 weeks, how often have you been bothered by any of the following problems? Little interest or pleasure in doing things; Feeling down, depressed or hopeless; Trouble falling or staying asleep, or sleeping too much" [44,45]. Responses for each question include, "Not at all;" "Several days;" "More than half the days;" and "Nearly every day." Scores range from 0 to 27. This variable was converted into a dichotomous variable with participants who screened negative (scores 0-9) and who screened positive (scores 10-27) for depression [44,45].
Anxiety was measured using the Generalized Anxiety Disorder 7-Item (GAD-7). This screening tool was developed to reflect all components of the Diagnostic and Statistical Manual for Mental Disorders (DSM)-IV criteria for generalized anxiety disorder and includes questions such as "Over the last 2 weeks, how often have you been bothered by the following problems? Feeling nervous, anxious or on edge; Not being able to stop or control worrying; Worrying too much about different things" [46]. Responses for each question include: "Not at all;" "Several days;" "More than half the days;" and "Nearly every day." Scores range from 0 to 21. This variable was converted into a dichotomous variable with participants who screened negative (scores 0-9) and those who screened positive (scores 10-21) for anxiety [46].
Eating disorders were measured using the 5-item SCOFF questionnaire [47]. The items include "Do you ever make yourself sick because you feel uncomfortably full? Do you worry that you have lost control over how much you eat? Have you recently lost more than 15 pounds in a 3-month period? Do you believe yourself to be fat when others say you are too thin? Would you say that food dominates your life?" A positive eating disorder screen was indicated by a score of two (2) or more "yes" responses [47,48]. This cut off score has been shown to have a sensitivity of 70-100% and specificity of 73-94% for anorexia nervosa, bulimia nervosa, and binge-eating disorder [47][48][49].
Suicidal ideation was measured using the question, "In the past year, did you ever seriously think about attempting suicide?" A dichotomous "yes" or "no" response was available. This item has been used in prior research [27,50].
Non-suicidal self-injury (NSSI) was measured using the question: "In the past year, have you ever done any of the following intentionally?" Potential responses include: "Cut myself;" "Burned myself;" "Punched or banged myself;" "Scratched myself;" "Pulled my hair;" "Bit myself;" "Interfered with a wound healing;" "Carved words of symbols into my skin;" "Rubbed sharp objects into my skin;" "Punched or banged an object to hurt myself;" and "Other." This variable was converted into a dichotomous variable with participants who reported any NSSI and those who reported none as has been done in prior research [27,42,51].

Demographic variables
Demographic variables included: self-reported age (range 18 to 79 years; 93.3% ≤ 30 years), race/ethnicity (White or Caucasian, non-Hispanic, non-Arab; Black or African American, non-Hispanic; Hispanic/Latino/a; Asian or Asian American; Arab/Middle Eastern or Arab American; American Indian, Alaskan Native, Native Hawaiian or Pacific Islander; other race/ethnicity; more than 1 race/ethnicity), sexual orientation (heterosexual; gay or lesbian; queer, questioning, or other), and highest parent education. Body mass index (BMI) was calculated based on self-reported height and weight (kg/m 2 ). In accordance with prior research on fasting and eating disorder behaviors [11,15,19,52], these demographic variables were adjusted for in the aim two analyses.

Statistical analysis
Descriptive analyses were conducted to characterize the sample. To determine our first aim, unadjusted prevalence of any and regular fasting by survey year were estimated among college men and women (Pearson chi-square tests). Multivariable logistic regression analyses were conducted to estimate the associations between the demographic variables and survey year as the independent variables, and any and regular fasting as the dependent variables, among college men and women. To determine our second aim, we pooled the survey years given the differing number of participants at each survey year (2016/2017: 1435; 2017/2018: 4352; 2018/2019: 2026; 2019/2020: 442), which increased statical power. Multiple logistic regression analyses were conducted to estimate the associations between any and regular fasting as the independent variables, and the substance use behaviors (cigarette use, marijuana use, other illicit drug use, alcohol use) and poor mental health symptoms (depression screen, anxiety screen, eating disorder screen, suicidal ideation, NSSI) as the dependent variables, while adjusting for the demographic variables. We also adjusted for survey year to account for any potential influence a single year may have had on the results. All analyses were stratified by gender given the potential differences in the purpose of fasting [19,21]. Statistical analyses included preconstructed sample weighting to adjust for nonresponse bias. Sample weights were constructed based on gender, race/ethnicity, academic level, and grade point average. Participants with underrepresented demographic characteristics are assigned greater sample weights [37]. Additional supplemental analyses (results stratified by year and BMI) are displayed in the online supplement. All analyses were conducted in 2021 using Stata 15.1 [53].

Results
Among the diverse sample of college student participants (Table 1), 67.59% (n = 5580) were women. The mean age and BMI for men were 22.96 (SE ± 0.17) years and 24.72 (SE ± 0.14), respectively, while the mean age and BMI for women were 22.31 (SE ± 0.12) years and 24.28 (SE ± 0.09), respectively. Roughly two thirds of both men (65.08%) and women (66.86%) in the sample identified as White or Caucasian, non-Hispanic, non-Arab. The majority of both men and women identified as heterosexual and had a highest parental education of some college or more. Women (18.12%; p < 0.001) had slightly higher prevalence of any fasting in the past 4 weeks, while there was no statistical difference in prevalence of regular fasting in the past 4 weeks among men (3.14%) and women (2.81%).
Aim one results: the prevalence and demographic correlates of fasting from 2016 to 2020 Results related to our first aim showed that, among both men and women, prevalence of any fasting in the past 4 weeks increased from 2016 to 2020 and was highest for both men (19.81%) and women (27.32%) in the 2019-2020 survey year. Any fasting increased 9.5 percentage points for men and 10.3 percentage points for women from 2016 to 2020. This increase was particularly significant among men (p < 0.001). Among both men and women, prevalence of regular fasting in the past 4 weeks significantly increased (p < 0.01) from 2016 to 2020 and was highest for men (4.66%) in the 2018-2019 survey year and women (6.19%) in the 2019-2020 survey year. Regular fasting increased 2.0 percentage points for men and 4.4 percentage points for women (Fig. 1).
Aim two results: associations between fasting, substance use behaviors, and symptoms of poor mental health Results from logistic regression analyses revealed significant associations between any and regular fasting and substance use behaviors and poor mental health symptoms (Table 4), although these patterns varied according to frequency of fasting and gender. Regarding reports of any fasting over the past 4 weeks, among men, any fasting was significantly associated with greater odds of a positive depression screen (AOR 3.40, 95% CI 2.53-4.56), a positive anxiety screen (AOR 2.73, 95% CI 1.98-3.78), a positive eating disorder screen (AOR 4.64, 95%   There were no significant associations between any fasting and alcohol use in the past 4 weeks. Regarding regular fasting in the past 4 weeks, among men, regular fasting was significantly associated with greater odds of a positive depression screen (AOR 3.70, 95% CI 2.07-6.62), a positive anxiety screen (AOR 4.32, 95% CI 2.32-8.04), a positive eating disorder screen (AOR 7.02, 95% CI 3.77-13.09), self-reported suicidal ideation in the past 12 months (AOR 3.00, 95% CI 1.43-6.26), and self-reported NSSI in the past 12 months (AOR 3.09, 95% CI 1.65-5.80) while adjusting for the demographic variables and survey year. There were no significant associations between regular fasting and the substance use measures.
Among women, regular fasting in the past 4 weeks was significantly associated with greater odds of any marijuana use in the past 30 days (AOR 2.14, 95% CI 1. 34-3.41), any other illicit drug use in the past 30 days (AOR 4.86, 95% CI 2.34-10.10), a positive depression There were no significant associations between regular fasting and any cigarette use in the past 30 days and alcohol use in the past 2 weeks. Overall, findings from the supplemental analyses were mostly similar with few differences when stratified by BMI (see Supplement).

Discussion
The aims of this study were to estimate the prevalence and demographic, substance use, and mental health correlates of fasting from 2016 to 2020 among a large, national sample of college men and women. The results showed that fasting has significantly increased from 2016 to 2020 among both men and women, with nearly 20% of men and 27% of women reporting fasting one or more times in the 2019-2020 survey year.
Results revealed unique demographic correlates of any and regular fasting among the sample. Among both men and women, higher BMI was associated with any fasting, which is not surprising given that a primary purpose of fasting is weight loss [1]; however, BMI was not associated with regular fasting. This finding my indicate that heightened risk for fasting is present among those with higher BMI solely when any fasting occurs, which may be due to the overall difficulty of maintaining fasting behaviors over many days within a four-week period. Among both men and women, sexual minority identity (e.g., gay or lesbian, bisexual, or queer, questioning or other) was associated with greater odds of any fasting, and greater odds of regular fasting was only among women. Prior research has shown that sexual minority young adults often engage in disordered eating behaviors at greater prevalence than their heterosexual peers, which may be interpreted in the context of minority stress theory [41,54]. Importantly, the bisexual men and women had the strongest association with any fasting, as well as regular fasting among women. This may be further evidence of the detrimental effects of "biphobia" [55]. Among men, identifying as multi-racial was associated with higher odds of any fasting. This may be attributed to the intersectional identities of gender and race/ ethnicity [56], as well as potentially unique appearance ideals [57]. Interestingly, among women, Hispanic/Latina identity was associated with lower odds of regular fasting, which may be evidence of stronger ethnic identity and lower eating disorder symptoms [57]. Additionally, among women, those with a highest parental education of some college or more was associated with lower odds of regular fasting. This finding, along with others related to educational attainment [58,59] may elucidate educational and class differences of regular fasting; however, there is conflicting evidence of the impact of education, both parental and self, as it relates to eating disorders [60].
Our results showed that both any and regular fasting was strongly associated with substance use behaviors among women and symptoms of poor mental health among both men and women. Among men and women, both any and regular fasting were associated with higher odds of all of the poor mental health symptoms assessed. However, the strength of the associations between fasting and symptoms of poor mental health were greater among those who reported regular fasting. This highlights the potentially detrimental mental health associations of regular fasting among college students. Furthermore, regular fasting may be a behavioral indicator of poor mental health among college students. These results highlight that psychiatric comorbidities remain common alongside eating disorder behaviors [30,61], and that men may experience similar or even greater levels of psychological impairment along with women [62,63].
While, overall, the patterns of poor mental health symptoms presented similarities across men and women, there were stark differences among substance use correlates. Among women, those who reported any fasting had greater odds of any cigarette smoking, marijuana use, and other illicit drug use. Interestingly, cigarette smoking was no longer associated with fasting among women who reported regular fasting, as opposed to less frequent fasting. Conversely, regular fasting was associated with nearly two-fold greater odds of marijuana use and nearly fivefold greater odds of other illicit drug use among women. While cigarette smoking [64] may be used to augment weight loss efforts given their appetite suppressant qualities, it may be that illicit drugs (e.g., stimulants, cocaine, ecstasy) are more effective for weight loss and suppressing appetite [65][66][67]. Relatedly, marijuana use is common among college age young adults [29], and fasting may be a mechanism to compensate for additional caloric intake given the common effect of increased appetite while under the influence of marijuana. These results among women further highlight the cooccurrence of substance use and eating disorders [30,68].
Interestingly, among men, there were no significant associations between any and regular fasting and any cigarette smoking, marijuana use, and other illicit drug use. In fact, among men, any fasting was associated with lower odds of alcohol use in the past 2 weeks. This may provide further evidence that fasting is a mechanism to improve psychological and physical performance [20,21], as well as reducing body adiposity to improve muscularity and leanness [61,69]. Thus, the use of substances would interfere with this intended outcome. Fasting has been shown to be a mechanism to compensate for the caloric intake from alcohol use [70,71], which these results indicate may not be true among men. This is further supported by sensitivity analyses exploring any and regular fasting and binge-drinking, among both men and women, which resulted in similarly null findings.
The results from this study have several important implications. Despite some of the benefits of fasting, particularly while under the advisement of clinical professionals, portrayed in prior research [1][2][3][4][5][6][7][8][9][10], the results from this study should caution individuals from engaging in this behavior, specifically those without appropriate oversight and supervision from a medical professional. Furthermore, the cultural and social promotion of fasting as a positive and effective behavior should be reassessed and minimized.
The results from this study also point to several theoretical implications and areas for future research. In general, fasting aligns more with traditional conceptualizations of drive for thinness and eating disorders among women [19,72] and occurs at greater prevalence among girls and women [73]. However, descriptively, women (18.12% vs. 14.77%) had higher prevalence of any fasting, while men (3.14% vs. 2.81%) had higher prevalence of regular fasting. This aligns with prior research showing that between 1.4-7.7% of boys and men, depending on age and timeframe, engage in fasting behaviors [73]. Furthermore, in this study, regular fasting was associated with over seven-fold greater odds of a positive eating disorder screen among men. These results indicate that traditional conceptualizations of fasting may be shifting, where, not only is the fasting behavior intended for optimizing performance [20,21], it is also associated with traditional eating disorder symptomology among men. It seems clear that the presentation of eating disorders, disordered eating, and performance-enhancing behaviors are complex among men and further research is needed to further conceptualize these behaviors to better describe their etiology.

Strengths and limitations
There are several important strengths to this study. These include the use of a national, large, and diverse sample of college men and women, as well as the use of several substance use and mental health measures. Despite these strengths, there are limitations to be noted. HMS is a cross-sectional survey, which limits the ability to draw inferences regarding causal relationships. This is particularly important given that it is highly likely that the relationships between fasting and substance use and poor mental health are bi-directional. Thus, future research is needed, particularly among a longitudinal cohort sample, to characterize the temporal relationships between these behaviors. Relatedly, given that HMS uses a module-based survey design, the number of participants who completed the module that included the fasting item differed from year to year (2016/2017: 1435; 2017/2018: 4352; 2018/2019: 2026; 2019/2020: 442), which may introduce selection bias. However, we adjusted for survey year in our analyses in an attempt to account for this potential bias. Furthermore, substantially more women than men were included in this study, which may impact generalizability of the findings. Survey items are based on self-report, which increases the potential for reporting bias; however, research has shown that young people are more willing to selfdisclose sensitive information in web-based surveys [74][75][76]. Relatedly, the fasting, substance use, and mental health variables were dichotomized for analysis, which may have oversimplified these behaviors and symptoms and impacted the results. Future research is needed to explore the breadth of substance use behaviors and poor mental health symptoms, including frequency, dose, intensity, and timing to further characterize the associations found in this study. Despite adjusting for several confounders (e.g., age, race/ethnicity) that may influence the relationship between the variables under study, there is the potential for unmeasured confounders. We were unable to conduct analyses among transgender and gender non-conforming college students due to a lack of statistical power. Lastly, we were unable to ascertain from the data the intensity, purpose, motivations, and true duration of the fasting behavior among participants. Thus, further research is needed to among the different demographic groups to add to our findings and further describe when and how fasting leads to substance use and poor mental health.

Conclusion
The purpose of this study was to determine the prevalence and demographic, substance use, and mental health correlates of fasting among college men and women. The results show that fasting is increasing among both men and women, with nearly one in five men and over one in four women reporting any fasting in the past 4 weeks. Any and regular fasting in the past 4 weeks were associated with key demographic correlates, including BMI, age, and sexual orientation. Furthermore, any and regular fasting in the past 4 weeks were associated with significantly greater odds of poor mental health symptoms among both college men and women and substance use behaviors among college women. Despite the purported benefits of fasting, as well as the social and cultural promotion in the media, there is cause for concern given these associations. Clinical professionals should screen for fasting and related psychopathology among young adults.